Tris Base CAS 77-86-1 Biological buffers manufacturer

Tris Base or THAM, is a white, crystalline powder with a slightly alkaline taste. It is used for biobuffer.

Tris Base Overview

Product Name: Tris Base

Chemical name: Tris(hydroxymethyl)aminomethane

Synonym: Tromethamine

CAS No.: 77-86-1

Molecular Formula: C4H11NO3

Molecular weight: 121.135

EINECS NO.: 201-064-4

Catalog: Biological buffers

Tris Base Properties

Use

Biochemistry and Molecular Biology

Tris base is a buffering agent in biological and biochemical research.

It helps maintain a stable pH level in solutions, particularly in the range of pH 7-9, which is suitable for many biological processes.

Tris buffers has common use in DNA and RNA isolation, electrophoresis, enzyme assays and protein purification.

Pharmaceuticals

In some pharmaceutical formulations,Tris is a pH adjuster and buffering agent.

It helps maintain the stability and effectiveness of certain over-the-counter drugs, including antacids and cough syrups.

Cell Culture and Biotechnology

Tris base is frequently a component of cell culture media and reagents. It helps regulate the pH of the culture medium,

providing an optimal environment for cell growth and viability. Tris also has use in the lysis buffers and protein extraction solutions.

Molecular Diagnostics

Tris is utilized in molecular diagnostic techniques such as polymerase chain reaction (PCR).

It includes in PCR buffer formulations to maintain the required pH for efficient DNA amplification.

Protein Electrophoresis

Additionally, Tris is commonly used in polyacrylamide gel electrophoresis (PAGE) systems for separating proteins.

It is a vital component of both the resolving gel and the stacking gel.

Tris helps establish the appropriate pH conditions for protein migration during electrophoresis.

Packaging

25kg/fiber drum

Storage

Store in a cool, dry environment, protected from moisture. Shelf life: 3 years.