MES monohydrate CAS 145224-94-8 , which is a buffering agent used in biochemistry and molecular biology that was selected and described.
MES is the common name for the compound 2-(N-morpholino)ethanesulfonic acid. Its chemical structure contains a morpholine ring.
MES monohydrate Overview
Product name : MES monohydrate
Synonym : 2-Morpholinoethanesulfonic acid monohydrate
CAS No.: 145224-94-8
EC Number : 224-632-3
Molecular Formula : C6H13NO4S*H2O
Molecular Weight : 213.25
Related products : MES CAS 4432-31-9 , MES hydrate 1266615-59-1
Category : Biological Buffers
MES monohydrate Properties
| Item | Specification |
| Appearance | White crystalline powder |
| Solubility (0.1M aq) | Clear , colorless solution |
| Assay | ≥ 99.5% |
| PH (1% aq) | 3.50 ~ 4.50 |
| Heavy metals | ≤ 5.0PPM |
| Water | 8.00 ~ 8.90% |
| Ultraviolet Absorbance 260nm (0.5M aq) | ≤ 0.025 |
| Ultraviolet Absorbance 280nm (0.5M aq) | ≤ 0.020 |
| pKa 25℃ | 5.9 ~ 6.3 |
MES monohydrate Use
MES monohydrate is used for biological buffers.
Biological and biochemical research
MES monohydrate maintains a stable pH environment in biological and biochemical experiments. It functions as a buffering agent, which helps to maintain a specific pH range during reactions or when works with sensitive biological samples.
Electrophoresis
MES monohydrate is a running buffer agent in electrophoresis, which is a technique used to separate and analyze molecules based on their charge and size. It helps to maintain the desired pH conditions for optimal separation and resolution of molecules during the process.
Cell culture
In cell culture applications, MES monohydrate is sometimes a buffering agent to control the pH of the growth media. It ensures a stable and suitable environment for the growth and viability of cells.
Enzyme assays
2-Morpholinoethanesulfonic acid monohydrate can be as a buffer in enzymatic assays, where it helps maintain the optimal pH range for enzyme activity. This allows for accurate measurement and analysis of enzyme kinetics.
Protein purification
During protein purification processes , MES monohydrate is a buffering agent in various purification steps. It helps to maintain the pH stability of the protein solution and prevents unwanted changes in protein structure or activity.
Packaging
25kg/fiber drum
Storage
Keep in cool and dry place, store in sealed containers. Shlf life : two years.
